PCR Equipment and Supplies
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Applied Biosystems™ TaqMan™ Multiplex Master Mix
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
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| Polymerase | AmpliTaq Fast DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2 X |
| Product Type | Multiplex Master Mix |
| Sample Type | DNA |
| Reaction Speed | Fast |
| Concentration | 2X |
| For Use With (Application) | Gene Expression,Virus Detection |
| PCR Method | Multiplex qPCR |
| Product Line | TaqMan |
| Detection Method | Primer-probe |
Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ PCR SuperMix
PCR SuperMix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
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Thermo Scientific™ Phusion Blood Direct PCR Kit
Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Phusion Blood II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Reaction Format | Separate Components |
| Product Type | Direct PCR Kit |
| Sample Type | Blood |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| Starting Material | DNA |
| PCR Method | Direct PCR |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Applied Biosystems™ TaqMan™ Genotyping Master Mix
Optimized for end-point fluorescence detection in SNP genotyping applications.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| GC-Rich PCR Performance | High |
|---|---|
| Fidelity (vs. Taq) | 2 X |
| Purity or Quality Grade | UP (Ultra Pure) |
| Concentration | 2X |
| For Use With (Application) | Genotyping |
| PCR Method | qPCR |
| Detection Method | Primer-probe |
| Polymerase | AmpliTaq Gold DNA Polymerase |
| Genotyping Target | Copy Number Variants, SNPs (Unknown or Numerous), Drug Metabolizing Enzymes, SNPs (Known) |
| Product Type | Genotyping Master Mix |
| Passive Reference Dye | ROX (Pre-mixed) |
| For Use With (Equipment) | Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 |
| Sample Type | DNA (Genomic) |
| Reaction Speed | Standard |
| Technique | SNP Genotyping (Real-Time PCR-Based), CNV (Copy Number Variation) |
| Product Line | TaqMan |
Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-CT™ Kits with sufficient reagents are provided for 100 reactions.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit
The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Format | Kit |
| Reverse Transcriptase | MultiScribe |
| Size (Final Product) | Up to 7 kb |
| Reaction Format | Premixed Components |
| Final Product Type | First-Strand cDNA |
| Reaction Speed | 30 to 60 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Yes |
| Optimal Reaction Temperature | 37°C |
| Technique | Reverse Transcription |
| Starting Material | RNA |
| For Use With (Application) | Real Time PCR (qPCR),RT-PCR |
Applied Biosystems™ Platinum™ Multiplex PCR Master Mix
The Platinum Multiplex PCR Master Mix is designed specifically for endpoint multiplex PCR.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 2.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,Multiplex PCR |
| Multiplex Capability | Up to 20-plex |
Invitrogen™ Cells-to-CT™ Stop Solution
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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A component of the Cells-to-CT™ Kits.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Cells-to-CT Bulk RT Reagents
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ Oligo(dT)20 Primer
Used for first-strand cDNA synthesis with reverse transcriptase at temperatures of ≥50°C, Oligo(dT)20 Primer is a string of 20 deoxythymidylic acid residues that hybridizes to the poly(A) tail of mRNA.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ OCT3/4 Recombinant Rat Monoclonal Antibody (EM92), Alexa Fluor™ Plus 488
Rat Recombinant Monoclonal Antibody
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Content And Storage | 4°C, store in dark, DO NOT FREEZE! |
|---|---|
| Target Species | Human |
| Host Species | Rat |
| Conjugate | Alexa Fluor Plus 488 |
| Applications | Flow Cytometry,Western Blot,Immunocytochemistry |
| Form | Liquid |
| Gene Accession No. | Q01860 |
| Isotype | IgG2a κ |
| Concentration | 1.0 mg/mL |
| Antigen | OCT3/4 |
| Gene Symbols | POU5F1 |
| Regulatory Status | RUO |
| Purification Method | Affinity chromatography |
| Gene Alias | 2-Oct; 3-Oct; 4-Oct; cb197; chunp6868; DADB-104B20.2; etID49452.21; gp9; gp-9; HGNC:9221; homeobox protein; lymphoid-restricted immunoglobulin octamer-binding protein NF-A2; MGC22487; NF-A3; Oct2; Oct-2; Oct2a; Oct2b; OCT3; Oct-3; Oct3/4; Oct-3/4; OCT4; Oct-4; Octamer-binding protein 2; octamer-binding protein 3; Octamer-binding protein 4; octamer-binding transcription factor 2; Octamer-binding transcription factor 3; OTF2; OTF-2; OTF3; Otf-3; Otf3g; Otf3-rs7; Otf4; Otf-4; OTTHUMP00000221150; OTTHUMP00000221151; POU class 2 homeobox 2; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU domain class 5 transcription factor 1; POU domain gene 2; POU domain protein 2; POU domain transcription factor OCT4; POU domain, class 2, transcription factor 2; POU domain, class 5, transcription factor 1; POU domain, class 5, transcription factor 3; pou2; pou-2; POU2F2; Pou5f1; pou5f1 protein; pou5f3; POU-type homeodomain-containing DNA-binding protein; spg; spg/pou2; spiel-ohne-grenzen; spiel-ohne-grenzen/pou2; wu:fd18d06 |
| Gene | POU5F1 |
| Product Type | Antibody |
| Gene ID (Entrez) | 5460 |
| Formulation | Proprietary buffer with 0.008% Bromonitrodioxane, 0.008% Methylisothiazolone; pH 6.8 |
| Classification | Recombinant Monoclonal |
| Primary or Secondary | Primary |
| Clone | EM92 |
Invitrogen™ Lambda Hind III dsDNA Markers
Generates fragments of the sizes 23,130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Invitrogen™ AccuPrime™ Pfx SuperMix
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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